The Economic Times

In 1992, Endeavour carried 4 female African clawed frogs into orbit; their eggs were fertilized in microgravity and developed into apparently normal tadpoles

In 1992, four frogs embarked on a journey aboard the space shuttle Endeavour as part of a groundbreaking reproductive experiment. Their eggs underwent fertilization in the microgravity of space, allowing scientists to observe early developmental challenges in space-flown embryos. Upon their return, however, the tadpoles displayed normal anatomical features and development, yielding vital insights for upcoming long-duration missions in space.